Non-human microbiome profiling (powered by Kepler)

Kepler maximizes the value derived from metagenomic data by blending the precision of K-mer exact-matching with the adaptability of probabilistic alignment. It accomplishes this by utilizing a curated, host-agnostic database of signature k-mers mapped across 30,000 species organized in a phylogenetic tree-like structure. This approach enables Kepler to achieve reliable identification and quantification of bacteria, viruses, fungi, and protists with leading speed and precision.

Kepler’s technology is at the heart of its innovation, holding patents in the US Patent office (US10108778B2, US20200294628A1) and the European Patent Office (ES2899879T3).

How Kepler Works

The Kepler multi-kingdom taxonomic profiler can be split into three parts:

Step 1. Harnessing a Curated Database of Microbial Genomes

Our Kepler database boasts a collection of meticulously curated microbial genomes, prioritizing high completeness, low contamination, and intra-species diversity. Through rigorous quality checks and selective curation, we ensure an optimal taxonomic signal-to-noise ratio, encompassing over 30,000 species spanning multiple microbial kingdoms.

Step 2. Identifying Relevant Signature Features

Within our curated genomes, a pre-computation phase dissects them into k-mers, categorizing them as shared or unique signature k-mers across taxa. Utilizing a phylogenetic tree-like structure, Kepler efficiently identifies shared and unique genomic signatures, essential for precise taxonomic classification.

Step 3. Searching the Taxonomy Database

During the computational phase, sequencing reads or contigs are meticulously compared against our database:

The first comparator employs k-mer sets to identify taxa present in the query, maintaining classification sensitivity and accuracy through composite statistics and coverage depth estimation.

The second comparator utilizes an edit distance-scoring probabilistic algorithm, ensuring precision and accuracy in taxonomic classification.

Evaluation of Kepler with Standardized Mock Communities

Benchmarking Kepler against leading profilers such as Kraken2/Bracken and MetaPhlAn4 using real-world community standards demonstrated its superiority. 

With exceptional F1-Scores, Kepler excels in detecting low-abundance taxa and differentiating closely related species, showcasing its precision even at the sub-species level.

For comprehensive insights and unparalleled microbial profiling, experience the power of the Kepler non-human profiler.

Contact us today to explore Kepler’s potential for non-human microbiome profiling.

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